Table 2.
Differentially methylated CpGs (DMCpGs) with a greater than 10% difference in methylation and a P-value < 0.05 for the difference between severe obesity cases and controls, as identified by the Human Methylation450 BeadChip array
| Gene Name | Probe | CHR | Genomic | P-value | Control | Obese |
|---|---|---|---|---|---|---|
| |
|
|
Co-ordinate |
|
|
|
| Hypomethylated DMCpGs | ||||||
| LRIG1* | cg26131019 | 3 | 66550740 | 4.96E-05 | 0.244 | 0.0723 |
| OIT3* | cg24769348 | 10 | 74692580 | 0.0002 | 0.4437 | 0.3271 |
| MGMT | cg09993319 | 10 | 131529400 | 0.0003 | 0.7305 | 0.5051 |
| PIWIL4 | cg 16436762 | 11 | 94307970 | 0.0004 | 0.5275 | 0.4228 |
| cg04456492 | 7 | 20010780 | 0.0007 | 0.802 | 0.6206 | |
| PCBD1 | cg05601623 | 10 | 72647650 | 0.001 | 0.228 | 0.1008 |
| cg23892028 | 6 | 27256340 | 0.0015 | 0.4042 | 0.2748 | |
| FER1L5* | cg20312012 | 2 | 97331030 | 0.0016 | 0.5836 | 0.4771 |
| AMBRA1* | cg20090290 | 11 | 46543700 | 0.0016 | 0.4844 | 0.3821 |
| A1CF | cg16531903 | 10 | 52645850 | 0.002 | 0.871 | 0.7431 |
| ARNT* | cg00944785 | 1 | 150825400 | 0.002 | 0.4981 | 0.3921 |
| FAM107B | cg14152591 | 10 | 14587920 | 0.0021 | 0.493 | 0.3628 |
| cg14192979 | 17 | 12562530 | 0.0025 | 0.4944 | 0.2951 | |
| cg26261358 | 15 | 24043140 | 0.0026 | 0.4387 | 0.2601 | |
| cg11557901 | 9 | 128022400 | 0.003 | 0.6408 | 0.5322 | |
| cg26576353 | 10 | 126135200 | 0.0033 | 0.8465 | 0.7393 | |
| TMCC1* | cg12196294 | 3 | 129575100 | 0.0033 | 0.6321 | 0.5305 |
| cg22626683 | 1 | 172903100 | 0.0037 | 0.7357 | 0.6279 | |
| PRG2 | cg15971518 | 11 | 57159180 | 0.0043 | 0.5815 | 0.393 |
| cg19699682 | 3 | 119349800 | 0.0045 | 0.552 | 0.425 | |
| B3GNT7 | cg00424152 | 2 | 232263100 | 0.0045 | 0.7712 | 0.6492 |
| IGF2BP3* | cg17209188 | 7 | 23387400 | 0.0048 | 0.5024 | 0.4001 |
| TAOK3 | cg17627898 | 12 | 118782500 | 0.0049 | 0.4411 | 0.3409 |
| cg07879897 | 17 | 66201170 | 0.005 | 0.2838 | 0.149 | |
| SULF2* | cg21130926 | 20 | 46415320 | 0.0054 | 0.6266 | 0.4702 |
| cg09196346 | 13 | 23499330 | 0.0054 | 0.2887 | 0.135 | |
| cg04450797 | 8 | 337367 | 0.0057 | 0.7987 | 0.6379 | |
| FSCN2 | cg05248234 | 17 | 79495520 | 0.0077 | 0.7439 | 0.6027 |
| cg11725581 | 7 | 6140990 | 0.0084 | 0.745 | 0.6439 | |
| AP3S1 | cg03637218 | 5 | 115209100 | 0.0087 | 0.7055 | 0.593 |
| ZNF827* | cg07091220 | 4 | 146752100 | 0.009 | 0.5674 | 0.467 |
| NAPSB | cg25094735 | 19 | 50848020 | 0.0091 | 0.6449 | 0.5334 |
| RIPK2* | cg15723028 | 8 | 90776470 | 0.0098 | 0.5502 | 0.4427 |
| PDLIM7 | cg15225325 | 5 | 176921800 | 0.0101 | 0.5126 | 0.3925 |
| SH3PXD2B | cg24921943 | 5 | 171847600 | 0.0102 | 0.5018 | 0.3943 |
| MIR125B1 | cg26916936 | 11 | 121970600 | 0.0116 | 0.6221 | 0.5164 |
| MATN2* | cg19935471 | 8 | 99048260 | 0.0116 | 0.6963 | 0.5957 |
| SLC35E2 | cg12213037 | 1 | 1666808 | 0.0122 | 0.7003 | 0.5419 |
| C18orf62 | cg23936477 | 18 | 73139740 | 0.0126 | 0.7775 | 0.6599 |
| PCDHB3 | cg23918315 | 5 | 140479000 | 0.0138 | 0.4064 | 0.2979 |
| RALGPS1* | cg14306650 | 9 | 129829100 | 0.0141 | 0.705 | 0.6032 |
| cg27114706 | 12 | 92527250 | 0.0148 | 0.877 | 0.7548 | |
| DNAJC8 | cg03040423 | 1 | 28560940 | 0.0157 | 0.9116 | 0.807 |
| MEGF11* | cg00682263 | 15 | 66188800 | 0.0163 | 0.5975 | 0.4801 |
| LOC652276* | cg06035616 | 16 | 2653306 | 0.0209 | 0.1801 | 0.0665 |
| RAB36 | cg05338731 | 22 | 23489040 | 0.0217 | 0.4992 | 0.3863 |
| cg00968488 | 5 | 103876200 | 0.0217 | 0.6865 | 0.5749 | |
| cg16885113 | 6 | 29648510 | 0.0235 | 0.8421 | 0.7381 | |
| AURKA | cg09712306 | 20 | 54949300 | 0.0248 | 0.481 | 0.3621 |
| cg23661721 | 14 | 95991370 | 0.0271 | 0.5536 | 0.4314 | |
| LOC652276 | cg03314158 | 16 | 2653280 | 0.0278 | 0.1674 | 0.0465 |
| AGPAT1 | cg17213381 | 6 | 32139500 | 0.029 | 0.8273 | 0.6971 |
| cg21852792 | 2 | 71678460 | 0.03 | 0.5511 | 0.4473 | |
| DEAF1 | cg03951394 | 11 | 660455 | 0.0316 | 0.8874 | 0.7094 |
| SORL1 | cg10746778 | 11 | 121460800 | 0.0341 | 0.5232 | 0.3801 |
| TOP1MT | cg00033213 | 8 | 144399300 | 0.0383 | 0.6014 | 0.4747 |
| cg06864789 | 6 | 139013000 | 0.0386 | 0.5387 | 0.3803 | |
| SNORA1* | cg09719956 | 11 | 93465910 | 0.0386 | 0.6563 | 0.5343 |
| cg05320460 | 15 | 63179550 | 0.039 | 0.7531 | 0.6475 | |
| cg27245056 | 10 | 122356500 | 0.0396 | 0.6572 | 0.5472 | |
| cg12799049 | 1 | 197811500 | 0.0401 | 0.4571 | 0.3469 | |
| CTSS | cg26891210 | 1 | 150703100 | 0.0402 | 0.7015 | 0.5925 |
| CBX7 | cg13306870 | 22 | 39527580 | 0.0408 | 0.3769 | 0.2444 |
| LTBP1* | cg11918450 | 2 | 33359200 | 0.0425 | 0.645 | 0.5413 |
| B4GALT6 | cg11986743 | 18 | 29205360 | 0.0447 | 0.6977 | 0.5903 |
| OTUD6B* | cg19586698 | 8 | 92097850 | 0.0463 | 0.7898 | 0.6607 |
| TP53INP2 | cg20592836 | 20 | 33292130 | 0.0496 | 0.5504 | 0.3536 |
| Hypermethylated DMCpGs | ||||||
| SPON2 | cg10852718 | 4 | 1167230 | 7.59E-06 | 0.734 | 0.8759 |
| cg24591913 | 10 | 47062880 | 0.0002 | 0.6864 | 0.8203 | |
| IL32* | cg16730716 | 16 | 3114986 | 0.0015 | 0.8161 | 0.9198 |
| FYN | cg26846943 | 6 | 112165100 | 0.002 | 0.2063 | 0.33 |
| TOX2 | cg26365090 | 20 | 42574360 | 0.0021 | 0.1902 | 0.4129 |
| TNXB | cg14188106 | 6 | 32063900 | 0.0021 | 0.4143 | 0.5163 |
| cg12342501 | 2 | 8530521 | 0.0021 | 0.6035 | 0.7998 | |
| cg27596172 | 8 | 26727370 | 0.0034 | 0.7664 | 0.8698 | |
| UBE4A | cg25574849 | 11 | 118269800 | 0.0035 | 0.2916 | 0.4022 |
| C7orf50 | cg26542892 | 7 | 1133730 | 0.0035 | 0.6345 | 0.7703 |
| TMEM71 | cg10054641 | 8 | 133773100 | 0.0041 | 0.3066 | 0.429 |
| cg08506672 | 5 | 3959743 | 0.0059 | 0.2318 | 0.4672 | |
| KRTAP27-1 | cg05809586 | 21 | 31709690 | 0.0063 | 0.5095 | 0.7153 |
| cg03240981 | 6 | 26615070 | 0.0063 | 0.4289 | 0.5301 | |
| cg00696044 | 10 | 63240300 | 0.0063 | 0.3894 | 0.5672 | |
| SLC12A8 | cg09866143 | 3 | 124861500 | 0.0078 | 0.565 | 0.7826 |
| WTIP* | cg10771931 | 19 | 34972140 | 0.0083 | 0.7332 | 0.8829 |
| SLC25A21 | cg00814218 | 14 | 37445440 | 0.0084 | 0.6818 | 0.8043 |
| CCDC88C | cg23165913 | 14 | 91880060 | 0.0086 | 0.413 | 0.5392 |
| CCS | cg24851651 | 11 | 66362960 | 0.0087 | 0.3717 | 0.701 |
| TRPM4 | cg01997599 | 19 | 49689670 | 0.0103 | 0.4019 | 0.5334 |
| ABCD3* | cg26908356 | 1 | 94929490 | 0.0111 | 0.4584 | 0.6008 |
| cg02479782 | 2 | 71033150 | 0.0113 | 0.3091 | 0.5286 | |
| cg08754654 | 5 | 154026400 | 0.0117 | 0.2817 | 0.3824 | |
| NAV1 | cg04287574 | 1 | 201619600 | 0.0124 | 0.2851 | 0.3895 |
| CSMD2 | cg21440084 | 1 | 34615960 | 0.0125 | 0.4898 | 0.6454 |
| cg10384133 | 9 | 45733080 | 0.0126 | 0.7488 | 0.8503 | |
| USP36 | cg01385356 | 17 | 76837380 | 0.0139 | 0.2224 | 0.3224 |
| cg07371521 | 5 | 154026400 | 0.0142 | 0.2003 | 0.3382 | |
| cg04653913 | 16 | 53407750 | 0.0145 | 0.588 | 0.6985 | |
| cg18618432 | 19 | 34621910 | 0.0147 | 0.5521 | 0.6704 | |
| TRAPPC9 | cg16191297 | 8 | 140926700 | 0.0156 | 0.7429 | 0.8567 |
| cg14580085 | 2 | 239553400 | 0.0172 | 0.5782 | 0.6973 | |
| cg24643105 | 11 | 113928500 | 0.0189 | 0.6603 | 0.7965 | |
| cg19373347 | 1 | 219634800 | 0.019 | 0.4338 | 0.5557 | |
| IL12RB1 | cg26642774 | 19 | 18170380 | 0.0191 | 0.5503 | 0.7219 |
| cg02389264 | 16 | 88757750 | 0.0191 | 0.6135 | 0.7415 | |
| cg10510935 | 1 | 4059661 | 0.0209 | 0.3138 | 0.4174 | |
| cg09916840 | 16 | 87248610 | 0.0214 | 0.0156 | 0.1245 | |
| cg02100397 | 19 | 646890 | 0.0223 | 0.4163 | 0.5673 | |
| SH3GL1 | cg11592634 | 19 | 4370001 | 0.0224 | 0.1313 | 0.3691 |
| C10orf110 | cg12421087 | 10 | 1083304 | 0.0225 | 0.6924 | 0.8347 |
| ANKH | cg14843632 | 5 | 14870590 | 0.0236 | 0.2696 | 0.3867 |
| ARPC3 | cg10738648 | 12 | 110888900 | 0.0238 | 0.5427 | 0.7118 |
| IRGM | cg22000984 | 5 | 150226300 | 0.0239 | 0.1801 | 0.3087 |
| SLC6A5* | cg14524936 | 11 | 20627600 | 0.0253 | 0.3286 | 0.4456 |
| cg01584086 | 11 | 10373720 | 0.0254 | 0.1171 | 0.2833 | |
| cg07700233 | 4 | 39171230 | 0.0264 | 0.3269 | 0.4351 | |
| ATP9B* | cg09636756 | 18 | 77134250 | 0.0267 | 0.7454 | 0.8943 |
| cg02088292 | 1 | 235099200 | 0.0268 | 0.2089 | 0.3511 | |
| RAB1A | cg00570635 | 2 | 65355270 | 0.0318 | 0.1747 | 0.3775 |
| SPDEF | cg16527629 | 6 | 34524700 | 0.0324 | 0.331 | 0.4785 |
| C14orf119 | cg08253809 | 14 | 23568020 | 0.033 | 0.1935 | 0.3079 |
| HADHA | cg01188578 | 2 | 26464060 | 0.034 | 0.2665 | 0.4863 |
| cg18182981 | 6 | 1449399 | 0.037 | 0.6137 | 0.7345 | |
| MOSC2 | cg12466610 | 1 | 220950200 | 0.0394 | 0.1258 | 0.3988 |
| FLJ42875 | cg08121686 | 1 | 2981840 | 0.0397 | 0.5734 | 0.714 |
| cg10588622 | 2 | 45397780 | 0.0421 | 0.2567 | 0.3627 | |
| SPTBN1 | cg01286930 | 2 | 54783770 | 0.0458 | 0.4365 | 0.5427 |
| cg07044115 | 10 | 123100200 | 0.0478 | 0.5947 | 0.6962 | |
| PMF1 | cg25465065 | 1 | 156198400 | 0.0486 | 0.5544 | 0.7344 |
| cg01118640 | 14 | 52816920 | 0.0494 | 0.6159 | 0.7683 | |
The genes tagged by “*”are associated with B lymphocytes, counted by the Houseman method, with P-value < 0.05 in line regression