Figure 1.
Targeting oncogenic KrasG12D expression and PEG3 deletion to the mouse thymus. (A) Upon Cre-mediated recombination: the allele housing the KrasG12D mutation (denoted by *12D) is conditionally expressed by removal of a tandem polyA signal. The critical exon 6 (denoted by yellow coloration) of Peg3 is conditionally deleted. Blue font denotes the allele is paternally inherited and pink font denotes the allele is maternally inherited. Gray boxes denote exons. Black triangles denote loxP sites. Filled in lollipops denote a methylated CpG island. Empty lollipops denote an unmethylated CpG island. (B) Breeding schematic from mating LSL-Kras+/G12D; Peg3+/del6 mice with MMTV-Cre mice to generate KPM, KM, PM, and M cohorts. (C) Specific PCR analysis of genomic DNA isolated from thymus confirms recombinant products for each of the targeted alleles.