Figure 3.

Lack of BER genes upregulation during TET-induced global DNA demethylation. (A) Western blot analysis showed no significant change of TDG, APEX1 and XRCC1 expression in D1 and mC3 clone cells and HEK293T parental cells after Dox treatment. All cells were harvested 3 days after Dox treatment at different dosages. (B) Constant expression levels of TDG, APEX1 and XRCC1 in D1 clone cells during 3 days of Dox treatment (2 μg/ml). The cell lysates were prepared every day after Dox treatment.