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. 2015 Nov 17;10(12):1143–1155. doi: 10.1080/15592294.2015.1114202

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Figure 4. — Prenatal immune activation increases 5-methylcytosine (5mC) binding at the GAD2 promoter region in the medial prefrontal cortex (mPFC). (A) 5mC binding was assessed by comparing the ratio of 5mC to the total cytosines in genomic DNA samples of control (CON) and poly(I:C) (POL) offspring. The bar plot shows 5mC ratios within the region of interest. *P < 0.05 as determined by a Student t-test. (B) 5hmC binding was assessed by comparing the ratio of 5hmC to the total cytosines in genomic DNA samples of CON and POL offspring. The bar plot shows 5hmC ratios within the region of interest. N(CON) = 8, N(POL) = 8. All values are means ± SEM.