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. 2014 Mar 3;3(1):143–159. doi: 10.3390/plants3010143

Figure 5.

Figure 5

Transcript levels of the representative JA-biosynthesis OsJAR1 (A) and OsAOC (B), and the JA-signaling gene OsJAZ8 (C) in wild type and mutant plants in response to wounding. Plants of wild type (grey bar) and phyAphyC (white bar) were wounded with MecWorm six weeks after germination for the indicated time intervals. qPCR analysis was carried out using two standard genes, eEF-1α and OsUBI5, for normalization. The fold change induction for each gene was calculated relative to a corresponding untreated control plant. Data represent the average of three independent experiments. Statistically significant differences to the wild type are indicated by an asterisk (Student’s t-test, p < 0.05).