Figure 3.

Chemotactic responses of pregametes (●) and gametes obtained from pregametes (○) towards 5 mM nitrite. Vegetative cells of mt⁻ were resuspended in TAP-N at time 0. The percentage of mating (Δ) is given for a comparison. At the times indicated, cells were mixed with an excess of sexually competent mating partner, and incubated in the dark. After 1h, the cells were fixed by the addition of glutaraldehyde and relative mating was determined as described in the Experimental Section. Data are means ± SD of triplicate determination from a representative experiment.