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. 2014 Feb 7;3(1):95–112. doi: 10.3390/plants3010095

Figure 4.

Figure 4

Quantitative RT–PCR results for OsIAA12, OsIAA29, OsINVINH3, OsLTPL32, OsDEFL13, ZmEBE-like Os11g18140, and OsHAP3D. Total RNA was extracted from developing rice grains 1–15 DAP using a Bioline ISOLATE Plant RNA Mini Kit. Each tube contained 50 ng RNA per 20 μL reaction and a final primer concentration of 0.5 μM. Amplification was carried out using a Bioline SensiFAST™SYBR No-ROX RT-PCR kit in a Rotor-Gene Q (QIAGEN) thermocycler fitted with a 100-tube rotor for 45 cycles. Controls (no reverse transcriptase) were included for OsINVINH3 and OsHAP3D. Expression was calculated relative to reference gene UBC using ROTORGENE software (QIAGEN). Melt curve analysis confirmed that a single product was obtained for each gene amplified. Results represent the means from three biological replicates ± standard error of the means.