Figure 3.

Effect of GA₃ on the expression of PA pathway genes. Relative fold expression of PA pathway genes in wheat grains exposed to GA₃ (60 μM) for 30, 45 and 60 min. After the exogenous application, the seeds were collected and RNA extraction was performed. The cDNA templates were prepared from 2 µg of DNase free RNA. Ct values obtained were normalized against Ta18SrRNA expression. Each bar indicates the mean of three to four replicates with the indicated standard deviation of the mean. ** indicates significant difference at p ≤ 0.01; * indicates significant difference at p ≤ 0.05 with respect to control.