Figure A1.

Semi-quantitative RT-PCR of wheat genes as a marker for ABA and GA₃ treatment on seeds. (A) PCR amplification of TaAGP-S (ADP-glucose pyrophosphorylase, smaller subunit) and TaGBSS1 (Granule-bound starch synthase 1) from 14 DAA seeds treated with ABA; (B) PCR amplification of TaBYM1 (β-amylase) and TaPHO-H (starch phosphorylase H) in wheat seeds (14 DAA) when treated with GA₃. After respective treatments the seeds were collected at 30 min, 45 min and 60 min post incubation. 2 µg of total RNA (DNase treated)was extracted and cDNA templates were prepared. Low-cycle semi-quantitative RT-PCR was performed along with Ta18SrRNA as an internal control. The amplicons were resolved on 1.2% agarose gel. The experiments were repeated twice from different biological replicates.