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. 2015 Apr 27;4(2):209–224. doi: 10.3390/plants4020209

Figure 2.

Figure 2

AON mutants and wild type plants fix equivalent amounts of nitrogen. Roots of A17, rdn1-2 and sunn-4 plants were evaluated 15 days after inoculation with S. medicae. Means ±se are per genotype (a) Mean nodule number (b) Mean number of nitrogen fixing (pink) nodules (c) Mean nodule fresh weight (ten samples of three nodules each from five to six plants per genotype). (d) Hydrogen production (μmol/hr) as a proxy for nitrogen fixation rate (see Experimental Section). Measurements were taken on the same plants at both 10 and 15 days after inoculation with S. medicae in two biological replicates. A17 (n = 9), rdn1-2 (n = 10) and sunn-4 (n = 9). Statistically significant differences from wild type based on Student’s t-test are indicated by * (p < 0.05) and ** (p < 0.01).