Figure 2.
The combination effect of α-Mangostin and retinoic acid was verified. (A, B) Within all these compounds, we found the effect of retinoic acid (10 μM) and verified its synergistic effect with α-Mangostin (5μM) in sk-mel-2 cells. The half-lethal dosage IC50 was decreased to 1.23 μM, nearly 8 times higher than the 10 μM in the single-agent group. (C) We determined the correlation of the synergistic effect with RAR level in RARα siRNA-transduced sk-mel-2 cells, showing that cells without RAR expression were not sensitive to retinoic acid, but the synergistic effect of retinoic acid and α-Mangostin was significantly improved.