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. 2016 Apr 22;22:1360–1367. doi: 10.12659/MSM.898204

Figure 3.

Figure 3

α-Mangostin activates retinoic acid-induced apoptosis. (A) Clonogenic survival assay was performed to examine the synergistic effect of the combination of retinoic acid and α-Mangostin on the survival of sk-mel-2 cells. Cell colonies were stained blue. (B) Colony numbers were counted. The paired t test was used to check the statistical significance (P<0.001). (C) The growth-inhibitory effects were examined via TUNEL staining. Slices were cultured in medium containing retinoic acid (2 μM), α-Mangostin (2 μM), or their combination for 3 h and stained for TUNEL (red) or 4′,6-diamidino-2-phenylindole (DAPI; blue) to label all cells. Representative images of 3 independent experiments are shown. D. The numbers of positive cell were counted. The paired t test was used to assess the statistical significance (P<0.001).