Table 1.
Amino Acid Composition (mole %) of the Root Cell Wall (CW), Insoluble Cell Wall Pellet Remaining after Treatment with HF (HFI), Trypsin (TI HFI) and Pronase (PI HFI).
| Amino Acid | WT | WT | WT | WT | ANP4 | ANP4 | ANP4 | ANP4 | ANP10 | ANP10 | ANP10 | ANP10 | MT | MT | MT |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CW | HFI | TI HFI | PI HFI | CW | HFI | TI HFI | PI HFI | CW | HFI | TI HFI | PI HFI | CW | HFI | TI HFI | |
| Asp | 6 | 1 | 2 | 1 | 6 | 6 | 4 | 4 | 6 | 4 | 1 | 1 | 7 | 5 | 5 |
| Glu | 6 | 3 | 2 | 2 | 5 | 4 | 3 | 3 | 6 | 3 | 2 | 2 | 7 | 2 | 4 |
| Hyp | 20 | 25 | 27 | 30 | 17 | 22 | 20 | 22 | 18 | 29 | 22 | 28 | 17 | 15 | 19 |
| Ser | 10 | 14 | 17 | 18 | 11 | 9 | 13 | 18 | 11 | 8 | 15 | 19 | 10 | 2 | 7 |
| Gly | 11 | 11 | 12 | 13 | 14 | 12 | 17 | 23 | 12 | 10 | 12 | 16 | 12 | 18 | 20 |
| His | 2 | 2 | 3 | 2 | 2 | 3 | 1 | 2 | 2 | 4 | 2 | 2 | 2 | 3 | 4 |
| Arg | 2 | 2 | 2 | 2 | 2 | 3 | 2 | 1 | 2 | 3 | 2 | 2 | 3 | 5 | 2 |
| Thr | 3 | 5 | 4 | 3 | 3 | 5 | 3 | 3 | 3 | 4 | 5 | 4 | 3 | 5 | 4 |
| Ala | 6 | 6 | 5 | 5 | 7 | 9 | 9 | 6 | 7 | 9 | 6 | 3 | 7 | 12 | 9 |
| Pro | 7 | 4 | 4 | 4 | 7 | 5 | 4 | 6 | 6 | 5 | 5 | 4 | 6 | 7 | 5 |
| Tyr | 4 | 2 | 2 | 2 | 3 | 2 | 3 | 2 | 3 | 2 | 1 | 2 | 3 | 2 | 1 |
| Val | 7 | 6 | 4 | 4 | 7 | 6 | 3 | 2 | 7 | 6 | 7 | 4 | 7 | 8 | 7 |
| Ile | 3 | 3 | 2 | 1 | 3 | 2 | 2 | 0 | 4 | 2 | 4 | 2 | 3 | 4 | 3 |
| Leu | 5 | 5 | 4 | 3 | 4 | 5 | 5 | 2 | 4 | 4 | 6 | 4 | 5 | 6 | 5 |
| Phe | 4 | 3 | 2 | 2 | 4 | 2 | 3 | 0 | 4 | 2 | 3 | 2 | 4 | 1 | 1 |
| Lys | 4 | 8 | 8 | 8 | 5 | 5 | 8 | 6 | 5 | 5 | 7 | 5 | 4 | 5 | 4 |
| Total | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| % Hyp 1 | 1.5 | 6.9 | 5.2 | 4.5 | 1.1 | 3.9 | 2.1 | 1.1 | 1.3 | 6.3 | 4.3 | 4.1 | 1.1 | 2.5 | 3.8 |
| % Protein 1 | 7.3 | 25.5 | 17.7 | 13.6 | 6.4 | 16.1 | 7 | 4.4 | 6.8 | 20.5 | 17.4 | 13.1 | 6.2 | 13.7 | 14.6 |
| Hyp/ Protein 2 | 0.2 | 0.27 | 0.29 | 0.33 | 0.17 | 0.24 | 0.3 | 0.25 | 0.19 | 0.31 | 0.25 | 0.31 | 0.18 | 0.18 | 0.26 |
| DW (mg) 3 | 50 | 10 | 8 | 7 | 38 | 10 | 7 | 6 | 51 | 10 | 8 | 7 | 4.6 | 1.6 | 0.75 |
| % Hyp Removed 4 | 6.7 | 39.7 | 54.3 | 10.0 | 62.3 | 83.1 | 7.1 | 45.4 | 54.4 | 10.0 | 10.9 | ||||
| % Protein Removed 4 | 30.1 | 44.5 | 62.3 | 34.4 | 69.6 | 83.6 | 41.1 | 32.1 | 55.3 | 20.0 | 60.9 |
1 % Hyp or Protein: the weight percentage of Hyp and total protein; 2 Hyp/Protein: weight ratio of Hyp in total protein content; 3 DW (mg): the dry weight of cell wall used in HF deglycosylation and the remaining HFI before and after trypsin/pronase digestion; 4 % Hyp or Protein removed: the weight percentage of Hyp and protein removed after HF, trypsin or, pronase digestion calculated from Hyp/protein weight percentage of stepwise HFI, TI HFI and PI HFI.