Table 2.
HRGPs Identified in WT and ANP Cell Wall HFI.
| Gene Accession # | Protein Identification (Plant Line of Origin) | Peptides Identified (Number of Occurrence in Protein Sequence; Peak of Peptide Identification) 1 | Peptide Mascot Ion Score 2 | Signal Peptide 3 | MM (kDa) 4 | pI 4 | |
|---|---|---|---|---|---|---|---|
| EXTs | |||||||
| AT1G76930 | EXT1 (WT, ANP4, ANP10) | YYSOOOVYK (3; W2, W6, W8, W10, WM3, WM13; FM16; A2) | 1–24 | 32.9 | 10.1 | ||
| HYSOOOVYK (9; WM6; A5), SOOOOVYK (1; FM8) | |||||||
| SOOOOVK (8; W1, W2, W5, WM22; AM3) | |||||||
| AT1G21310 | EXT3, RSH (WT) | HYSOOOVYHSOOOOK (13; WM6), HYTOOVK (1; WM4), | 1–28 | 49.2 | 10.3 | ||
| SOOOOVK (13; W1, W2, W5, WM22) | |||||||
| AT3G54580 | EXT17 (ANP4) | SOOOOYYSOSOK (15; FM8) | 54.9 | 1–22 | 105.7 | 9.5 | |
| AT2G43150 | EXT215 (WT) | SOOOOYYYHSOOOOVK (6; W8, W10) | 54.3 | 1–27 | 23.4 | 9.6 | |
| SOOOOVK (10; W1, W2, W5, WM22) | |||||||
| AT1G12040 | LRX1 (ANP4, ANP10) | LQGPLPSSVGNMK (1; F3) | 42.3 | 1–26 | 81 | 8.2 | |
| LLYELDLSNNR (1; AM4) | 44.5 | ||||||
| AT1G62440 | LRX2 (ANP4, ANP10) | SLEQLNVANNR (1; FM4, FM12, FM13) | 71.4 | 89.8 | 4.8 | ||
| LSGPLPSSIGNMK (1; A2) | 59.4 | ||||||
| AT4G13340 | LRX3 (ANP10) | FNEFEGTVPK (1; AM5, AM13, AM15, AM20) | 65.9 | 1–20 | 82.2 | 7 | |
| AT3G22800 | LRX6 (ANP4) | SLEQLNIAHNK (1; FM6) | 55.7 | 1–28 | 52.3 | 6.2 | |
| AT3G50580 | HAE2 (ANP10) | SOOOOTOK (1; AM3, AM12) | 41.7 | 1–24 | 27.2 | 9.1 | |
| AGPs | |||||||
| AT2G33790 | AGP30 (WT) | LPAYPOAK (1; W2), TLVAVR (1; WM3), VSSLHDGGK (1; WM4) | 1–25 | 25.8 | 10.6 | ||
| AT1G28290 | AGP31 (WT, ANP4, ANP10) | SLVAVR (1; W8, W10, WM3), SOVKPOVK (1; WM4, WM14), | 1–25 | 38.4 | 10.8 | ||
| NITAETTTDK (1; FM8), | |||||||
| AOVSOOAK(O/P)(O/P)VK(O/P)(O/P)VY(O/P)(O/P)TK7 | |||||||
| (1; W3, W4, W7, W8, W10, WM3, WM18, WM19, WM22; F1, F4, FM4, FM16; A5, AM1), LFGGDVGAELKPEK | |||||||
| (1; FM7, FM8, FM18; AM10, AM11, AM18) | |||||||
| AT2G04780 | FLA7 (WT) | FTDVSGTVR (1; W10, WM3) | 61.4 | 1–22 | 26.8 | 6.5 | |
| AT3G52370 | FLA15 (WT) | HHFNGEAQVK (1; WM24) | 42.3 | 1–20 | 48.1 | 6.7 | |
| AT2G35860 | FLA16 (ANP4) | EETOATEIKPAAOVVK (1; F1, FM4, FM13) | 55.4 | 1–23 | 49.1 | 6.8 | |
| PRPs | |||||||
| AT4G38770 | PRP4 (ANP4) | EVPO(P/O)VOVYK(P/O)(P/O)(P/O)K7 (1; F1), | 1–29 | 49.1 | 10.3 | ||
| IEHPO(P/O)VOVYKOO(P/O)K7 (1; F2, FM18) | |||||||
| AT5G09530 | PRP10 (WT, ANP4, ANP10) | FPENSKPEVPK (1; WM8, WM9; F2, FM18; A5) | 64.8 (in WT) | 1–35 6 | 41.6 | 5.9 | |
| VPEIPKPEETK (1; FM4, FM11, FM12) , MPEIQKPELPK | |||||||
| (1; FM4, FM12, FM13), LPDIPK (1; FM16), | |||||||
| LPEVPK (1; FM16), LPEFPKPELPK (1; AM4, AM18), | |||||||
| MPEIPKPELPK (1; AM20) |
1 Only peptides that pass the 95% Scaffold confidence filter are shown; 2 The Mascot ion score are listed for those HRGPs that only have one peptide that pass the 95% Scaffold confidence filter but with score > 40; 3 Signal peptides were predicted by SignalP (www.cbs.dtu.dk/services/SignalP); 4 Predicted protein molecular weight (MM) and isoelectric point (pI) values were obtained from TAIR (www.arabidopsis.org); 5 EXT21 shares one peptide (SOOOOVK) with EXT1 and EXT3, however the Mascot ion score of its unique peptide SOOOOYYYHSOOOOVK is larger than 40, thus validates the existence of EXT21; 6 For PRP10, SignalP prediction indicated the absence of a signal peptide while the cellular localization predicted by TargetP (http://www.cbs.dtu.dk/services/TargetP/) indicated a secretory pathway localization and thus the possession of a signal peptide. The signal peptide sequence and the putative cleavage site were determined according to the S-score (signal sequence score) and C-score (cleavage score) by SignalP; 7 The hydroxylation of some of the Pro residues in some AGP31 and PRP4 peptides could not be precisely determined, thus both possible sequences (either Pro-Hyp or Hyp-Pro) are indicated as P/O.