. 2015 Jan 21;4(1):85–111. doi: 10.3390/plants4010085

Table 3.

HRGPs Identified in MT Cell Wall HFI.

Gene Accession #	Protein Identification	Peptides Identified ¹ (Number of Occurrence in Protein Sequence)	Peptide Mascot Ion Score ²	Total Spectra Count ³	Signal Peptide ⁴	MM (kDa) ⁵	pI ⁵
EXTs
AT1G76930	EXT1	HYSOOOVYK (9), YYSOOOVYK (3)		6	1–24	32.9	10.1
AT1G21310	EXT3, RSH	HYSOOOVYHSOOOOK (13)	46.6	1	1–28	49.2	10.3
AT2G43150	EXT21	SOOOOYYYHSOOOOVK (6)	54.4	3	1–27	23.4	9.6
AT1G12040	LRX1	KVTVFDITSNR (1), VTVFDITSNR (1), VVLSLPSLK (1)		5	1–26	81	8.2
AT1G62440	LRX2	ELGLLTDLALFHLNSNR (1), FPNVVLSLPSLK (1), SLEQLNVANNR (1)		10		89.8	4.8
AT4G13340	LRX3	DLDAIFINHNR (1), FNEFEGTVPK (1), FPTVVLQLPSLK (1)		11	1–20	82.2	7
AT4G13340	LRX3	FRFELPENFGDSPVSVIVLANNR (1)		11	1–20	82.2	7
AT3G24480	LRX4	FPTVVLHLPSLK (1), SAYIALQAWK (1)		11	1–25	54.7	6.9
AT4G18670	LRX5	FAGIFPTVVLQLPSLK (1), FNEFEGPVPR (1)		9	1–31 ⁶	90.8	6.7
AGPs
AT1G28290	AGP31	LFGGDVGAELKPEK (1), NGYFLLLAPK (1), TVTNFGFR (1)		5	1–25	38.4	10.8
AT2G25060	PAG2	LSLVVISPR (1)	44.7	1	1–28	19.5	7.4
PRPs
AT3G62680	PRP3	GLTGVPLALYGYR (1), SNTEVVIYSNPTDSK (1)		2	1–22	34.4	9.7

¹ Only peptides that pass the 95% Scaffold confidence filter are shown; ² The Mascot ion score are listed for those HRGPs that only have one peptide that pass the 95% Scaffold confidence filter but with score>40.; ³ Total spectra count indicates the relative abundance of protein in the sample; ⁴ Signal peptides were predicted by SignalP; ⁵ Predicted protein molecular weight (MM) and isoelectric point (pI) values were obtained from TAIR; ⁶ For LRX5, SignalP prediction indicated the absence of a signal peptide while the cellular localization predicted by TargetP indicated a secretory pathway localization and thus the possession of a signal peptide. The signal peptide sequence and the putative cleavage site were determined according to the S-score and C-score by SignalP.