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(A) Determination of the T-DNA insertion numbers by Southern blot hybridization with a DIG-labeled GAL4 specific probe. Numbers of DIG-labeled fragments indicate numbers of T-DNA insertions in the different enhancer trap lines; (B) T-DNA insertion site and direction of the reporter element (GFP, green arrow) and the coding sequences of the flanking genes (red arrows) in the line N9313.
