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. 2014 Sep 5;3(3):442–457. doi: 10.3390/plants3030442

Figure 1.

Figure 1

The dynamic morphology of tonoplast intrinsic proteins (TIP)3-GFP-labelled protein storage vacuoles (PSVs) during 4 days of germination. (A) TIP3-GFP labels spherical PSVs in the aleurone layer of mature kernels, plus occasional ellipsoid PSVs (arrows); (B) Homogeneous and tightly packed PSVs after one day of germination; (C) Arrows indicate PSVs starting to form buds at 2 days after germination; (D,E) PSVs become larger and more heterogeneous, with spherical, ellipsoid, small and large structures. Arrows indicate that small PSVs are still connected to large ones. Scale bar = 5 µm. (F) The average PSV size in µm during 4 days of germination. Standard error bars are indicated (n = 100).