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Expression and purification of recombinant amidases. Hexahistidine tagged amidases were expressed in E. coli and purified as described in the experimental section. Aliquots of 12.5 μL of the corresponding elution fractions were used for SDS-polyacrylamide gel electrophoresis and subsequent coomassie-staining (A) and immunoblotting (B). For the immunoblot only the region between 75 and 37 kDa is shown.
