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. 2016 Jan 9;2(3):317–327. doi: 10.1016/j.jcmgh.2015.12.010

Figure 1.

Figure 1

Identification of small-molecule CFTR activators. (A) Project overview. (B) CFTR activator screen using FRT cells co-expressing human wild-type CFTR and YFP iodide–sensing protein. Test compounds at 10 μmol/L were added for 10 minutes at room temperature in the presence of forskolin (125 nmol/L) before iodide addition. Examples of data from single wells of a 96-well plate showing CFTR activation by CFTRact-J027 are shown. (C) Structures of CFTR activators emerging from the screen. (D) Synthesis of CFTRact-J027. DMF, dimethylformamide.