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. 2013 Apr 24;2(2):248–278. doi: 10.3390/plants2020248

Table 1.

Pollen performance traits measured for this study and methods used to determine trait values. Ls = style length (mm); Cx,y = number of callose plugs present in the interval between x and y, where x = distance from the stigma-style junction (mm); y = x + 1 mm; C0,1 = number of callose plugs present 0–1 mm from the stigma-style junction; t = time between pollination and style harvest (hours). See the Experimental Section for additional details.

Trait Definition Determination of trait value Styles used to determine trait value
Pollen germination A binary variable signifying pollen grain germination and initial pollen tube entry into the style. We concluded that pollen germination had occurred if we observed at least one pollen tube in the stigma-style junction 2.5 hours following pollination. All styles sampled
Pollen tube growth rate, PTGR (mm/hour) Mean speed by which pollen tubes travel through the style 1. Estimate mean pollen tube length (mm), Lp
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Styles in which at least one pollen tube was observed at the stigma-style junction 2.5 hours post-pollination
2. Divide by t to estimate pollen tube growth rate (PTGR)
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Proportion of the entire style length traveled per hour Mean proportion of the style length traversed per hour 1. Divide PTGR by the length of the style in which it was estimated.
Inline graphic
Styles in which at least one pollen tube was observed at the stigma-style junction 2.5 hours post-pollination