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. 2013 Apr 29;2(2):302–316. doi: 10.3390/plants2020302

Figure 5.

Figure 5

Molecular analyses of gene expressed in five dry seed samples representing four other plant species. The five seed samples are numerically labeled as 1: peanut cv. 4401, 2: maize cv. Tien Khao, 3: maize cv. Tien Dam, 4: sunflower cv. Beauty Mix, and 5: Jasmine rice cv. KDML105. (A) PCR amplification of EF1-alpha gene based on the templates obtained from 1st-stranded cDNA, genomic DNA and total RNA; these three templates are labeled with the letters S, G and R, respectively. (B) the agarose gel showing the SRAP-cDNA amplifications using three primer combinations following Mornkham et al. [26] from 1st-stranded cDNAs produced from extracted seed RNAs. (C) qPCR amplification of a 105 bp fragment of the EF1-alpha gene for five seed samples using HtEF1_F and HtEF1_R primers. (D) amplification curves of the PCR products for EF1-alpha gene. (E) melting peaks of the PCR products for EF1-alpha gene.