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. 2013 Aug 9;2(3):507–520. doi: 10.3390/plants2030507

Figure 4.

Figure 4

PIRL6 gene structure, T-DNA insertion alleles, and knockout status. (A) The PIRL6 locus (At2g19330), with exons shown as blue rectangles, introns and flanking regions as lines. Positions of T-DNA insertions in putative knockout mutants pirl6-1 (SAIL574A05) and pirl6-2 (WISCDSLOX393-396L14) are indicated by arrows. Horizontal arrows indicate positions of RT-PCR primers used for part B; (B) Detection of PIRL6 transcripts in pirl6-1 and pirl6-2 putative T-DNA knockout lines. RT-PCR was carried out with PIRL6-specific primers on total RNA isolated from flowers of homozygous mutant (p6-) or wild-type (WT) plants. G, PIRL6 gene (with introns) amplified from genomic DNA. RT-PCR specificity was confirmed by sequencing of gel purified reaction products.