Figure 5.

Up-regulation of SAGs in osphyB-2 mutants during DIS rescued by supplementation with KNO₃. Detached leaf segments from one-month-old WT (W; black bars) and osphyB-2 (p; white bars) plants grown under LD conditions were floated on the 3 mM MES (pH 5.8) buffer without (−KNO₃) or with 1.9 g/L KNO₃ (+KNO₃), with the abaxial side up at 28 °C in darkness, and were sampled at 0 (control) and 3 DDI for RT-qPCR. RT-qPCR analysis was used to measure the relative transcript levels of OsABI5 (A); OsNAP (B); OsSGR1 (C); OsNYC1 (D); OsATG5 (E); OsATG7 (F); OsATG8a1 (G); and OsATG12 (H); and their transcript levels were normalized to the transcript levels of OsUBQ5. Mean and SD values were obtained from more than three biological replicates. These experiments were repeated twice with similar results. Asterisks indicate significant difference between WT and osphyB-2 (Student’s t-test p values, * p < 0.05; ** p < 0.01). DDI, day(s) of dark incubation; NS, not significant.