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. 2015 Sep 1;4(3):644–663. doi: 10.3390/plants4030644

Figure 6.

Figure 6

Expression of six rice PIF genes during DIS. Detached leaf segments from one-month-old WT and osphyB-2 plants grown under LD conditions were incubated on the 3 mM MES (pH 5.8) buffer with the abaxial side up at 28 °C in darkness, and were sampled at 0 to 4 DDI for RT-qPCR. RT-qPCR was used to measure the relative transcript levels of OsPIL11 (A); OsPIL12 (B); OsPIL13 (C); OsPIL14 (D); OsPIL15 (E); and OsPIL16 (F); and transcript levels were normalized to the transcript levels of OsUBQ5. Black and white bars indicate WT and osphyB-2, respectively. Mean and SD values were obtained from more than three biological replicates. Asterisks indicate significant difference between WT and osphyB-2 (Student’s t-test p values, * p < 0.05; ** p < 0.01). These experiments were repeated twice with similar results. DDI, day(s) of dark incubation; NS, not significant.