Table 1.
Overview of LTP genes analyzed in this study.
| 10 dai | 23 dai | Locus | Description | Name | LTP Mutants Used | Fold-Regulation of Transcript Levels for Altered LTP Gene |
|---|---|---|---|---|---|---|
| −50.0 | 445.5 | AT2G38540 | Non-specific lipid transfer protein | LTP1 | LTP1-OX | 2.6 ↑ |
| −1.2 | 87.1 | AT5G59320 | Predicted to encode a PR (pathogenesis-related) protein. Belongs to the lipid transfer protein (PR-14) family | LTP3 |
LTP3-OX LTP3-KO |
4.2 ↑ 20.3 ↓ |
| 1.2 | 539.2 | AT5G59310 | Encodes a member of the lipid transfer protein family | LTP4 |
LTP4-OX LTP4-KO |
10.5 ↑ 3.5 ↓ |
| −4.8 | −14.3 | AT1G12090 | Extensin-like protein |
AT1G12090-OX AT1G12090-KO |
1.5 ↑ 2.7 ↓ |
|
| −2.7 | −10.0 | AT2G18370 | Predicted to encode a PR (pathogenesis-related) protein. Belongs to the lipid transfer protein (PR-14) family | LTP8 |
LTP8-OX LTP8-KO |
4.6 ↑ 8.6 ↓ |
| 1.2 | −25.0 | AT5G05960 | Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein | AT5G05960-KO | 20.9 ↓ | |
| −3.2 | −14.3 | AT3G22620 | Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein | AT3G22620-KO | 1.6 ↓ | |
| 4.5 | 4.6 | AT4G33550 | Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein | AT4G33550-AS | 10.3 ↓ | |
| −1.5 | 103.1 | AT1G62510 | Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein | AT1G62510-AS | 4.7 ↓ |
The Table shows the regulation of LTP gene expression in response to P. brassicae infection 10 days after inoculation (10 dai) and 23 days after inoculation (23 dai). Data from a microarray [11] were re-evaluated for LTP genes. Additionally the LTP mutant lines (M) used in this study are listed, whereas OX indicates overexpression of the gene; KO indicates the gene disruption due to T-DNA insertion leading to reduced gene expression (knockdown) or complete repression of gene expression (knockout) and AS indicate gene silencing via antisense RNA. The transcript analyses for the mutant lines were done using semi-quantitative RT-PCR and the program Image J to calculate the regulation of transcript levels by determining the intensity of the respective PCR product.