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. 2016 Jan 8;5(1):5. doi: 10.3390/plants5010005

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Effects of free linolenic acid (LnA) on insect elicitor (IE) induced jasmonic acid accumulation; (B) Effects of free linolenic acid (LnA) on mechanical wounding (MW) induced jasmonic acid accumulation; (C) Comparison of volatiles release from corn seedlings pre-treated LnA and then with IE. Controls were treated similarly, but without LnA. Plant were treated with 300 μM aqueous solution of the respective fatty acid overnight and then treated by either MW or application of IE. JA was measured 1 and 2 h after treatment. JA was analyzed by GC/MS. Plant volatiles were collected between 4 and 5 h after treatment with IE [34]. Different letters above each bar indicate statistical difference determined by ANOVA analysis followed by Tukey tests where appropriate (p < 0.05). N = 5, error bars represent standard deviation. Statistical analysis was done for each volatile group independently.

(A) Effects of free linolenic acid (LnA) on insect elicitor (IE) induced jasmonic acid accumulation; (B) Effects of free linolenic acid (LnA) on mechanical wounding (MW) induced jasmonic acid accumulation; (C) Comparison of volatiles release from corn seedlings pre-treated LnA and then with IE. Controls were treated similarly, but without LnA. Plant were treated with 300 μM aqueous solution of the respective fatty acid overnight and then treated by either MW or application of IE. JA was measured 1 and 2 h after treatment. JA was analyzed by GC/MS. Plant volatiles were collected between 4 and 5 h after treatment with IE [34]. Different letters above each bar indicate statistical difference determined by ANOVA analysis followed by Tukey tests where appropriate (p < 0.05). N = 5, error bars represent standard deviation. Statistical analysis was done for each volatile group independently.