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. 2015 Dec 22;5(1):1. doi: 10.3390/plants5010001

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunodetection of large (LSU; (a)) and small (SSU; (b)) subunits of Rubisco, D1 protein of the photosystem II (c) and lhcb3 of the light harvesting complex (d) in the source leaves in response to restricted (LN, 0.375 mM) or ample (HN, 3.75 mM) nitrate applied for 21 days. The protein extracts of the three biological repetitions were pooled and 15 µg were loaded per lane for immunodetection. The protein abundance was determined with specific antibodies (see Experimental Section for details). D0: day 0; D14: day 14; D21: day 21. In LN conditions, immunodetection was not performed at D21 because there was not enough protein. The variation in protein abundance (ΔD21 or ΔD14) between D0 and D21 or D0 and D14 (when D21 is not available) is expressed as a percentage of the abundance observed at D0 and is given on the right of each immunoblot.