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. Author manuscript; available in PMC: 2016 Apr 25.
Published in final edited form as: Mol Microbiol. 2015 Nov 13;99(3):484–496. doi: 10.1111/mmi.13241

Fig. 6. Topoisomerase genes are not regulated by the master late regulator EUO.

Fig. 6

A. In vitro transcription assay showing that EUO did not repress the three topoisomerase promoters. For each gene, a transcription templates containing the promoter region was transcribed with E. coli RNA polymerase in the absence or presence of EUO. The EUO target omcAB promoter and the EUO-independent dnak P1 promoter were used as positive and negative controls respectively.

B. Graph of the effect of EUO on transcriptional activity. For each promoter, transcription in the presence of EUO was normalized to baseline transcription in the absence of EUO (defined as 100%). Values are from the average of at least three independent experiments with standard deviation indicated by the error bar.