Fig. 3.

Effect of pharmacological HDAC 3 inhibition on the (pro)inflammatory gene expression profile in mouse precision-cut lung slices. Schematic representation of the experimental setup and timeline (A). Mouse precision-cut lung slices (PCLS) were subjected to RGFP966 and SAHA for 20 h and stimulated with LPS/IFNγ for the last 4 h of the experiment and subsequently lysed. Inflammatory gene expression was assessed by real-time PCR and expressed as fold change compared to control (LPS/IFNγ-treated) group (B). Effect of HDAC 3 inhibition on M2 marker gene expression in PCLS is shown in (C). Data are presented as mean values ± SD; n = 3–5. ^*p < 0.05; ^**p < 0.01 compared to vehicle. N.D. = not determined for SAHA.