Fig. 6.

HDAC 3-selective inhibitor RGFP966 reduces the protein expression of HDAC 1 and HDAC 2 in RAW 264.7 macrophages. RAW 264.7 macrophages were incubated with RGFP966, stimulated for 4 h with LPS/IFNγ and subsequently harvested. Gene expression of NF-kB p65, IκBα, HDAC 1, HDAC 2 and HDAC 3 was analyzed by real-time qPCR (A). Data are presented as mean values ± SD; n = 3–4. In parallel, the effect of RGFP966 on total NF-kB p65 and HDAC 1–3 protein expression in LPS/IFNγ-stimulated RAW 264.7 macrophages was analyzed by immunoblotting (B) and quantified by densitometric analysis (C). Protein levels were normalized against β-actin and control (LPS/IFNγ-treated) cells were set at 100%. Data are presented as mean values ± SD of 4 independent experiments and a representative blot is shown in B. ^*p < 0.05; ^**p < 0.01 compared to vehicle.