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. Author manuscript; available in PMC: 2016 Apr 25.
Published in final edited form as: Mol Cancer Res. 2015 Feb 4;13(4):636–650. doi: 10.1158/1541-7786.MCR-13-0268

Figure 3.

Figure 3

Effects of JMJD3 expression on cell morphology, cytoskeleton, and growth characteristics. (A) Phase-contrast images of U251.vector, U251.JMJD3mut, and U251.JMJD3wt cells. Note the differences in cell morphology, growth density and numbers of cells with anchorage independence growing above the monolayer (arrows). Percentages of cells in each field showing cell-cell contacts and presence of cortical F-actin: U251.vector, 78.3% contacts, 12.3 cortical actin; U251.JMJD3mut, 98.8% contacts, 2.6% cortical actin; U251.JMJD3mut, 23.7% contacts, 38.3% cortical actin. Scale bar = 200 μm. (B) Alexa488-conjugated phalloidin visualization of F-actin (red) in U251 cells transfected with the indicated constructs. In B2, nuclei are counterstained with DAPI (blue). Note the decreased cell contact and presence of cortical (plasma membrane-associated) F-actin in the U251.JMJD3wt cells. Scale bar = 50 μm. (C) Phase-contrast microscopy of parental (untransfected) U87 cells, and U87.JMJD3mut and U87.JMJD3wt cells. (D) Alexa488-conjugated phalloidin visualization of F-actin (red) in U87 cells transfected as indicated. Scale bar = 200 μm. D2 shows enlarged areas of D1 (scale bar = 50 μm).