Figure 3.
(A) Composite light microscopy image of half of a gut cross-section. Arrows point to the “pocket structures” where the symbionts were observed. Lu = gut lumen. Scale bar, 100 μm. (B) TEM micrograph of partially-visible elongated bacterial cells among host microvilli within pockets formed by the gut epithelium. Arrows point to various bacteria. Scale bar, 2 μm. (C) TEM micrograph close-up of three bacteria, one almost fully visible. Arrows point to the bacteria. Scale bar, 2 μm. (D) Extended focus multi-channel epifluorescence micrograph of a gut section hybridized with the symbiont-specific probe PricSym652-CY3 and stained with DAPI. Cand. T. priapulorum symbionts appear red, host nuclei cyan, and autofluorescent host tissue yellow. Scale bar, 5 μm.
