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. 2016 Apr 26;10:106. doi: 10.3389/fncel.2016.00106

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Copyright © 2016 Heise, Schroeder, Schoen, Halbedl, Reim, Woelfle, Kreutz, Schmeisser and Boeckers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Colocalization of Shank puncta with VGLUT1 puncta in the intragranular mossy fibers and the stratum moleculare of the dentate gyrus. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: dentate Gyrus = DG, containing stratum granulare = SG, stratum moleculare = SM, and polymorphic layer = PL; I. indicates uppermost row/figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) and VGLUT1 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 μm), the bottom rows (small rectangles) show further enlargements (indicated in upper row by white rectangles) in the PL (1, top row) and SM (3, bottom row) (scale bar = 5 μm).