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Quantitative image analysis of HER2, CK5/6, and ER by CRi Nuance software
| Marker | Images (n) | Total signal OD values (×103) | T value | P value | |
|---|---|---|---|---|---|
| RGB images | MS images | ||||
| HER2 | 540 | 87.510 ± 39.885 | 99.833 ± 50.829* | −13.557 | 0.000 |
| CK5/6 | 420 | 69.917 ± 26.210 | 76.205 ± 29.771* | −12.641 | 0.000 |
| ER | 480 | 64.879 ± 24.932 | 70.989 ± 27.958* | −13.482 | 0.000 |
Data were presented as means ± standard deviation
OD optical density
* P < 0.05 compared with RGB images
