Figure 6. Consecutive hyper-osmotic stresses result in uncorrelated transcription events.

(a) Evolution of the NaCl concentration over time. Cells were stimulated at time 0 with a given concentration of NaCl. A second hyper-osmotic stress of similar amplitude was performed 43 min later by doubling the concentration of NaCl in the well: 0.1→0.2 (orange), 0.15→0.3 (cyan), 0.2→0.4 (blue) (throughout the entire figure). (b,c) Quantification of the average Hog1 nuclear accumulation (b) and pSTL1-dPSTR^R nuclear enrichment (c) for cells subjected to the steps in NaCl concentrations depicted in (a) (0.1→0.2: N_C=429; 0.15→0.3: N_C=450; 0.2→0.4: N_C=449). (d) Quantification of the average pGPD1-dPSTR^R nuclear enrichment, from a different strain, subjected to the steps in NaCl concentrations depicted in a (0.1→0.2: N_C=235; 0.15→0.3: N_C=296; 0.2→0.4: N_C=276). These cells also bear Hog1-YFP that showed the same behaviour as in b. (e–g) Correlations of the signalling outputs (e), of the expression outputs of pSTL1-dPSTR^R (f) or of the expression outputs of pGPD1-dPSTR^R (g) for the two stress events.