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. 2016 Apr 21;7:11394. doi: 10.1038/ncomms11394

Figure 6. Wound-recruited ILC3s produce IL17F.

Figure 6

(a) Cytokine production profile of CD45+Lin−CD127+RORγ+ILC3s assessed by flow cytometry with intracellular staining. Total skin cells unstimulated or stimulated for 3 h in the presence of PMA and ionomycin were analysed. Numbers indicate the percentage of cells in respective quadrants. Analysed cells pooled from eight biological replicates. IgG1a isotype antibodies were included as controls for the intracellular staining. (b) mRNA was isolated from unwounded (0 dpw, n=5) or wounded RORγ+/− (n=6) skin collected 2 dpw. Relative mRNA levels of IL22 were quantified by qRT-PCR. mRNA levels were normalized to unwounded controls (designated 1). Data plotted using a quartile, box and whisker plot. Data were not normally distributed and the medians of experimental replicates were analysed by Mann–Whitney test. Wounding induced statistically significant upregulation of IL22 (***, P=0.0170).