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. 2016 Apr 22;7:11358. doi: 10.1038/ncomms11358

Figure 1. MLCK and RLC phosphorylation are required for the basal tone in IAS.

Figure 1

(a) Time courses of changes in force after application of 0.5 g tension in CTR and MlckSMKO mice. (b) Summarized data showing much smaller IAS tone in MlckSMKO mice than in control (mean±s.e.m., CTR n=7, MlckSMKO n=5, ****P<0.0001 by two-tailed Student's t-test). (c) Faeces from CTR mice and MlckSMKO mice after 20 days of tamoxifen treatment. (d) The length (left) and width (right) of faeces were increased in MlckSMKO mice compared with the controls. Bars represent mean±s.e.m., n=20, ****P<0.0001 by two-tailed Student's t-test. (e) Examples of RLC phosphorylation during the process of spontaneous tone generation in IAS from CTR and MlckSMKO mice. (f) Quantification of p-RLC during basal tone generation in CTR and MlckSMKO IAS. Bars represent mean±s.e.m., n=3, *P<0.05, **P<0.01, ***P<0.001 by two-tailed Student's t-test.