FIG 2.
Multiplex iciHHV-6A/B ddPCR sensitively and simultaneously detects HHV-6B and ciHHV-6A in serum samples from four individual HCT recipients. Sera of ciHHV-6A HCT recipients were tested for reactivation of HHV-6A (squares) and HHV-6B (open circles). Each point represents the mean for 3 ddPCRs. The dashed line at 102 copies/ml represents the limit of detection for HHV-6B. Each asterisk (*) denotes a sample positive for HHV-6B by qualitative real-time PCR. (A, C, and D) Three individual HCT recipients with donor-derived ciHHV-6A; (B) HCT recipient with related donor- and recipient-derived ciHHV-6A.