TABLE 1.
Description of mutations in ompK36 in K. pneumoniae isolates
| Isolate by bla gene carried | MIC (mg/liter) |
ompC/K36 mutationb | OmpK36c | Other bla gene(s)d | |
|---|---|---|---|---|---|
| ETP | CAZa | ||||
| blaIMP | |||||
| JIE533 | 32 | NA | Premature stop codon. 181delG. Val61fsTer11 | No | blaTEM |
| blaDHA | |||||
| JIE2597 | 64 | NA | IS903B, after nucleotide 137 | No | blaSHV-5/12 |
| JIE3813 | 64 | NA | Premature stop codon. 91_92insA. Leu31fsTer7 | No | blaCTX-M-15, blaOXA-30 |
| blaCTX-M-15 | |||||
| JIE1309 | 8 | NA | ISKpn26, 48 bp upstream ATG (46) | No | |
| JIE1398e | 8 | NA | Premature stop codon. 374G→A. Trp125Ter | No | blaTEM, blaOXA-30 |
| JIE1462 | 8 | NA | Gly134_Asp135dup | Yes | blaTEM, blaOXA-30 |
| JIE1474 | 8 | NA | Gly134_Asp135dup | Yes | blaTEM, blaOXA-30 |
| JIE1505e | 16 | NA | IS903, after nucleotide 126 | No | blaTEM |
| JIE1652 | 8 | NA | Premature stop codon. 262delA. Arg88fsTer7 | No | blaTEM |
| JIE1703 | 8 | NA | Ser332Pro | No | blaOXA-30 |
| JIE1983 | 4 | NA | Premature stop codon. 371_383del. Ser124fsTer38 | No | blaOXA-30 |
| JIE2038 | 8 | NA | Gly134_Asp135dup | Yes | blaTEM, blaOXA-30 |
| JIE2055 | 8 | NA | Gly134_Asp135dup | Yes | blaTEM, blaOXA-30 |
| JIE2157 | 64 | NA | 1009_1016del. Ile337fs | No | blaOXA-30 |
| blaCTX-M-14 | |||||
| JIE1333 | 64 | 16 | Gly134_Asp135dup | Yes | blaTEM, blaOXA-48 |
| JIE1334 | 64 | 16 | Gly134_Asp135dup | Yes | blaTEM, blaOXA-48 |
| JIE1335 | 64 | 16 | Gly134_Asp135dup | Yes | blaTEM, blaOXA-48 |
| JIE1348 | 64 | 32 | Gly134_Asp135dup | Yes | blaTEM, blaOXA-48 |
| JIE1383 | 64 | 8 | Gly134_Asp135dup | Yes | blaTEM, blaOXA-48 |
| JIE1482 | 64 | 32 | Gly134_Asp135dup | Yes | blaTEM, blaOXA-48 |
| JIE2218 | 128 | 16 | Gly134_Asp135dup | Yes | blaTEM, blaOXA-48 |
| JIE2999e | 64 | 256 | Premature stop codon. 508C→T. Gln170Ter | No | |
| JIE2753 | 1 | 8 | Val10Glu | No | blaTEM |
CAZ MIC is only valid to predict ompK36 defects in isolates carrying blaCTX-M-14. NA, not applicable.
ompK36 mutations. For the premature stop codon, mutations are described at the DNA and protein levels, respectively. ins, insertion; del, deletion; dup, duplication; →, substitution; fs, frameshift; Ter, stop codon. For example, for JIE533, 181delG. Val61fsTer11 indicates that there is a deletion of glutamine at position 181, causing a frameshift where valine 61 is the first amino acid affected, creating a new reading frame ending with a stop codon at position 11 (counting starts with the valine as amino acid 1). Val10Glu denotes that amino acid Val10 is changed to Glu.
SDS-PAGE analysis: yes, OmpK36 detected by SDS-PAGE; no, OmpK36 not detected by SDS-PAGE.
Genes detected by mPCR/RLB (16, 17) but not sequenced. blaOXA-30 indicates a blaOXA-30/1-like gene. blaOXA-48 indicates a blaOXA-48-like gene.
ompK35 was sequenced in these isolates. JIE1398 and JIE2999, intact OmpK35 sequence identical to accession no. WP_004141771.1. JIE1505, premature stop codon. 706insA. Tyr236fsTer2.