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. Author manuscript; available in PMC: 2017 Apr 13.
Published in final edited form as: FEBS Lett. 2016 Apr 13;590(8):1042–1052. doi: 10.1002/1873-3468.12144

Fig. 4.

Fig. 4

Charge-based phosphomimetics decrease Fyn activation loop phosphorylation for the Tyr440Asp allele (A–B) and increase activation loop phosphorylation for the Y3D allele (C–D). The indicated Fyn alleles were expressed in HEK 293 cells and whole cell extracts were subjected to SDS-PAGE and immunoblotting with the indicated antibodies. The Log2 fold change in activation loop phosphorylation relative to the expression of individual alleles was determined by densitometry. The means of three experiments normalized to wild type levels are indicated as well as the individual data points and comparisons of statistical significance. †For this KD replicate pY416 levels were not above background. A very conservative noise level of 7.5 fold lower than WT was used for the calculation, giving a value ~1/3 the mean of the other two KD values.