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. Author manuscript; available in PMC: 2017 Apr 13.
Published in final edited form as: FEBS Lett. 2016 Apr 13;590(8):1042–1052. doi: 10.1002/1873-3468.12144

Fig. 5.

Fig. 5

Charge-based phosphomimetics in Fyn's SH2 domain increase Fyn kinase activity as measured by total tyrosine phosphorylation in whole cell extracts. The indicated Fyn alleles were expressed in HEK 293 cells and whole cell extracts were subjected to SDS-PAGE and immunoblotting with the indicated antibodies. The Log2 fold change in tyrosine phosphorylation of cellular substrates above 70 kDa relative to the expression of individual alleles was determined by densitometry. The means of three experiments (one for Tyr185Asp) normalized to wild type levels are indicated as well as the individual data points and comparisons of statistical significance.