Table 1.

Autophosphorylation sites identified following the expression of the indicated human SFKs in yeast. Following cell lysis, extracts were digested with trypsin and tryptic peptides were immunoprecipitated with anti-phosphotyrosine antibodies. Retrieved peptides were subjected to LC-MS/MS. “Yes” indicates the site was found phosphorylated by mass spectrometry. “Yes?” at Tyr214 indicates the phosphorylation site could not unambiguously be distinguished from the Tyr213 equivalent. “No” indicates the site was not identified as phosphorylated, and “Phe”, “Leu”, or “Ser” indicate these non-tyrosine residues were found in the homologous position. Human Fyn numbering is used on the top row for reference. Homologous site in the other kinases are in each column.

	Y185	Y213	Y214	Y420	Y440
Yes	No	No	Yes	Yes	Yes
Fyn	Yes	Yes	Yes	Yes	Yes
Fgr	No	Yes	Yes	Yes	Phe
Lyn	Phe	Yes	Yes?	Yes	Phe
Hck	No	Phe	Yes	Yes	Phe
Blk	Phe	Yes	Yes	Yes	Phe
Frk	Phe	Phe	Phe	No	Ser
Srm	Yes	Leu	Yes	Yes	Yes