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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Free Radic Biol Med. 2016 Feb 16;94:145–156. doi: 10.1016/j.freeradbiomed.2016.02.012

Table 1.

Molecular mass of Prdx6 proteins determined by sedimentation equilibrium analysis (experimental mass) and mass calculated from the amino acid composition

Experimental Mass Calculated Mass*
Monomer Dimer
Daltons Daltons
Wild Type (WT) 51,060 24,904 49,808
Oxidized WT† 25,016/49,232# 24,920 49,840
L145E 24, 813 24,920 49,840
L148E 24,254 24,920 49,840
*

Mass is calculated for the sulfenic acid form for oxidized WT and for the reduced form for the other proteins using the average atomic mass for each element.

†

Prdx6 was treated with 60 mM H2O2 for 1 h at room temp.

#

Both species present.