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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Free Radic Biol Med. 2016 Feb 16;94:145–156. doi: 10.1016/j.freeradbiomed.2016.02.012

Table 4.

Effect of mutation at L145 and/or L148 generated through infection of Prdx6 null MPMVEC with lentiviral vectors on cellular peroxidase and PLA2 activities

Enzymatic activity nmol/h/mg protein
Peroxidase PLA2
H2O2 PLPCOOH
Vector 7990 ± 220 60 + 12 0.02 ± 0.01
WT 14750 ± 60 774 ± 42 7.85 ± 0.13
L145E 9530 ± 440 (23%) 197 + 84 (19%) 7.17 ± 0.15 (91%)
L148E 9380 ± 260 (21%) 117 + 18 (8%) 7.28 ± 0.23 (93%)
L145/148E 7810 ± 147 (0%) 73 + 6 (2%) 6.88 ± 0.23 (88%)

Activities were measured in cell lysates and are expressed per h of incubation. Peroxidase activity was measured with H2O2 or 1- palmitoyl -2- linoleoyl- sn- glycerol -3- phosphocholine hydroperoxide (PLPCOOH) as substrate. PLA2 activity was measured as in Table 3. Values are mean ± SE for n = 3. The number in parenthesis indicates the % of the WT value after subtracting the baseline (vector). Previously reported values for wild type cells under control conditions are 816±95 nmol/h/mg protein for peroxidase activity (PLPCOOH substrate) and 9.28±0.52 nmol/h/mg protein for PLA2 activity (12).