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. Author manuscript; available in PMC: 2016 May 26.
Published in final edited form as: Mol Membr Biol. 2015 May 26;32(2):39–45. doi: 10.3109/09687688.2015.1023378

Figure 1.

Figure 1

(A) Representative western analysis of protein lysates prepared from isolated brain capillaries from matched wild-type and hAPP mice at age 3 months, and (B) capillaries of wild-type mice before and after challenge with 100nM human Aβ40. (C) Purification profile by metal affinity chromatography of human ABCB1. Lanes 1, membrane fraction (100μg of protein or 0.25% of volume); 2, solubilised membrane fraction (0.25%); 3, column flow-through (0.25%); 4-13, washes from 20 mM to 200 mM imidazole in 20 mM increments (each 4% of volume); 14, EDTA 50 mM (4% of volume). (D) Western analysis of purified ABCB1 before and after ubiquitination. NEDD4-1 reaction mixture in the absence (lane 1) and presence (lane 2) of methylated ubiquitin.