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. Author manuscript; available in PMC: 2016 May 26.
Published in final edited form as: Mol Membr Biol. 2015 May 26;32(2):39–45. doi: 10.3109/09687688.2015.1023378

Figure 3.

Figure 3

Flow cytometric analysis of ABCB1 in the plasma membrane of Flp-In-ABCB1-12His cells. Left dotplot, cells were gated for normal size and granularity (the same gate was used for populations); Middle dotplot, two colour analysis of mock-transfected cells; Right dotplot, two colour analysis of cells transfected transiently with pcDNA3-NEDD4-1-mCherry. Each dot represents a single cell and the density of cells is colour coded from blue (few cells) to red (many cells). The numbers represent the percentage of cells in a given gate, and the coloured letters define the analytical gates used to plot the histogram. The histogram shows ABCB1 expression in NEDD4-1-expressing (orange and red) and non-expressing cells (blue and green). Histogram colour coding is consistent with the lettering in the dotplots.