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. Author manuscript; available in PMC: 2016 Apr 26.
Published in final edited form as: Cell Metab. 2012 Apr 4;15(4):451–465. doi: 10.1016/j.cmet.2012.02.013

Figure 4. Molecular regulation of ROS-dependent extension of life span following daf-2 impairment.

Figure 4

(A) Oxygen consumption, (B) mitochondrial ROS levels in aak2(ok524) nematodes following exposure to RNAi against daf-2 (black bars) relative to effects on control RNAi-treated aak2(ok524) nematodes (white bars) at different time points. In both panels relative values are depicted; for absolute values, see Figure S3. (C) Lifespan analyses of aak-2(ok524) nematodes exposed to control-RNAi (open circles) in the presence (red) or absence (black) of the antioxidant NAC; life span analyses of aak2(ok524) nematodes exposed to RNAi against daf-2 (closed triangles) in the presence (red) or absence (black) of NAC. (D) Lifespan analyses in the presence of mutation and RNAi as in C, replacing NAC with the antioxidant BHA (blue). (E) Lifespan analyses of pmk-1(km25) nematodes exposed to control-RNAi (open circles) in the presence (red) or absence (black) of NAC; life span analyses of pmk-1(km25) nematodes exposed to RNAi against daf-2 (closed triangles) in the presence (red) or absence (black) of NAC; (F) Lifespan analyses in the presence of mutation and RNAi as in E, replacing NAC with BHA (blue). (G) Lifespan analyses of skn-1(zu67) nematodes exposed to control-RNAi (open circles) in the presence (red) or absence (black) of NAC; life span analyses of skn-1(zu67) nematodes exposed to RNAi against daf-2 (closed triangles) in the presence (red) or absence (black) of NAC. (H) Lifespan analyses in the presence of mutation and RNAi as in G, replacing NAC with BHA (blue). Activities of superoxide dismutase (I) and catalase (J) in wild-type nematodes and mutants for pmk-1 and skn-1 without (white bars) and with (black bars) daf-2 RNAi treatment for 5 days. In panels I and J relative values are depicted; for absolute values, see Figure S3. (K) Lifespan analyses of N2 nematodes exposed to control RNAi (empty circles) and daf-2 RNAi (black triangles) in comparison to exposure against sod-3 RNAi (purple circles) alone and co-incubation with daf-2 RNAi (purple triangles). (L) Lifespan analyses of N2 nematodes exposed to control RNAi (empty circles) and daf-2 RNAi (black triangles) in comparison to exposure against ctl-2 RNAi (purple circles) alone and co-incubation with daf-2 RNAi (purple triangles). (M) Lifespan analyses of daf-16(mu86) nematodes exposed to control-RNAi (open circles) in the presence (red) or absence (black) of NAC; lifespan analyses of daf-16(mu86) nematodes exposed to RNAi against daf-2 (closed triangles) in the presence (red) or absence (black) of NAC. (N) Lifespan analyses in the presence of mutation and RNAi as in M, replacing NAC with BHA (blue). In panels A, B, I and J values are given as mean ± SD. *p<0.05, **p<0.01, ***p<0.001 versus respective controls.