Table 1.
Life Span Assay Results and Statistical Analyses
| Strain, RNAi, Solvent | Maximum Life Span (d) (+/− SEM) | Mean Life Span (d) (+/− SEM) | P-value (vs. control, see footnotes) | Number of experiments (n) | Number of nematodes (n) |
|---|---|---|---|---|---|
| N2 (control RNAi) H2O | 28.0 +/−0.6 | 19.40 +/−0.3 | 3 | 323 | |
| N2 (control RNAi) NAC/H2O | 27.3 +/−0.9 | 18.96 +/−0.2 | n.s.a | 3 | 357 |
| N2 (daf-2 RNAi) H2O | 70.3 +/−0.9 | 46.76 +/−1.0 | <0.0001a,b | 3 | 310 |
| N2 (daf-2 RNAi) NAC/H2O | 60.3 +/−0.9 | 39.64 +/−1.1 | <0.0001a,b,c | 3 | 318 |
| N2 (control RNAi) DMSO | 31.0 +/−0.6 | 19.89 +/−0.2 | 3 | 335 | |
| N2 (control RNAi) BHA/DMSO | 28.3 +/−0.9 | 19.32 +/−0.2 | n.s.d | 3 | 331 |
| N2 (daf-2 RNAi) DMSO | 71.7 +/−1.2 | 46.00 +/−1.0 | <0.0001d,e | 3 | 342 |
| N2 (daf-2 RNAi) BHA/DMSO | 60.0 +/−0.6 | 34.16 +/−0.7 | <0.0001d,e,f | 3 | 331 |
| daf-2(e1370) H2O | 73.5 +/−1.5 | 51.09 +/−0.6 | 2 | 218 | |
| daf-2(e1370) NAC/H2O | 61.5 +/−1.5 | 44.87 +/−0.5 | <0.0001g | 2 | 248 |
| daf-2(e1370) DMSO | 73.5 +/−1.5 | 47.23 +/−1.1 | 2 | 222 | |
| daf-2(e1370) BHA/DMSO | 63.0 +/−2.0 | 31.81 +/−1.2 | <0.0001h | 2 | 253 |
| N2 (control RNAi) | 30.0 +/−0.5 | 21.0 +/− 0.1 | 2 | 386 | |
| N2 (sod-3 RNAi) | 30.0 +/−1.0 | 20,6 +/− 0.2 | n.s.a | 2 | 412 |
| N2 (daf-2 RNAi) | 72.0 +/−0.5 | 42.1 +/− 1.2 | <0.0001a | 2 | 352 |
| N2 (sod-3 RNAi/daf-2 RNAi) | 55.0 +/−1.9 | 37.1 +/− 0.7 | <0.0001a,c | 2 | 394 |
| N2 (ctl-2 RNAi) | 30.0 +/−1.0 | 20.8 +/− 0.2 | n.s.a | 2 | 452 |
| N2 (ctl-2 RNAi/daf-2 RNAi) | 61.0 +/−2.4 | 40.1 +/− 0.8 | <0.05a,c | 2 | 427 |
| age-1(hx546) H2O | 50.5 +/−0.5 | 28.90 +/−0.2 | 2 | 247 | |
| age-1(hx546) NAC/H2O | 41.0 +/−1.0 | 23.46 +/−0.2 | <0.0001i | 2 | 256 |
| age-1(hx546) DMSO | 50.5 +/−1.0 | 27,87 +/−0.2 | 2 | 259 | |
| age-1(hx546) BHA/DMSO | 44.5 +/−0.5 | 22,63 +/−1.6 | <0.0001k | 2 | 242 |
| aak-2(ok524) (control RNAi) H2O | 24.5 +/−0.5 | 16.78 +/−0.4 | 2 | 213 | |
| aak-2(ok524) (control RNAi) NAC/H2O | 24.0 +/−1.0 | 16.65 +/−0.3 | n.s.a | 2 | 234 |
| aak-2(ok524) (daf-2 RNAi) H2O | 36.0 +/−1.0 | 21.02 +/−0.1 | <0.0001a,b | 2 | 229 |
| aak-2(ok524) (daf-2 RNAi) NAC/H2O | 38.0 +/−1.0 | 21.13 +/−0.3 | <0.0001a,b,, n.s.c | 2 | 231 |
| aak-2(ok524) (control RNAi) DMSO | 24.0 +/−1.0 | 16.64 +/−0.4 | 2 | 217 | |
| aak-2(ok524) (control RNAi) BHA/DMSO | 24.0 +/−0.0 | 16.89 +/−0.7 | n.s.d | 2 | 220 |
| aak-2(ok524) (daf-2 RNAi) DMSO | 37.5 +/−1.5 | 22.53 +/−0.2 | <0.0001d,e | 2 | 202 |
| aak-2(ok524) (daf-2 RNAi) BHA/DMSO | 37.5 +/−0.5 | 23.16 +/−0.3 | <0.0001d,e,, n.s.f | 2 | 205 |
| pmk-1(km25) (control RNAi) H2O | 28.0 +/−1.0 | 19.36 +/−0.2 | 2 | 178 | |
| pmk-1(km25) (control RNAi) NAC/H2O | 29.5 +/−0.5 | 19.42 +/−0.2 | n.s.a | 2 | 160 |
| pmk-1(km25) (daf-2 RNAi) H2O | 38.0 +/−1.0 | 27.02 +/−1.1 | <0.0001a,b | 2 | 166 |
| pmk-1(km25) (daf-2 RNAi) NAC/H2O | 55.0 +/−2.0 | 34.13 +/−1.0 | <0.0001a,b,c | 2 | 170 |
| pmk-1(km25) (control RNAi) DMSO | 26.5 +/−1.5 | 17.49 +/−0.7 | 2 | 157 | |
| pmk-1(km25) (control RNAi) BHA/DMSO | 28.5 +/−1.5 | 17.40 +/−0.6 | n.s.d | 2 | 161 |
| pmk-1(km25) (daf-2 RNAi) DMSO | 41.5 +/−1.5 | 28.16 +/−1.2 | <0.0001d,e | 2 | 148 |
| pmk-1(km25) (daf-2 RNAi) BHA/DMSO | 54.0 +/−2.0 | 32.97 +/−1.2 | <0.0001d,e, =0.00010f | 2 | 151 |
| skn-1(zu67) (control RNAi) H2O | 21.5 +/−0.5 | 16.77 +/−0.1 | 2 | 140 | |
| skn-1(zu67) (control RNAi) NAC/H2O | 22.5 +/−0.5 | 16.57 +/−0.1 | n.s.a | 2 | 160 |
| skn-1(zu67) (daf-2 RNAi) H2O | 34.0 +/−1.0 | 21.89 +/−0.1 | <0.0001a,b | 2 | 144 |
| skn-1(zu67) (daf-2 RNAi) NAC/H2O | 45.5 +/−0.5 | 26.71 +/−1.1 | <0.0001a,b,c, | 2 | 150 |
| skn-1(zu67) (control RNAi) DMSO | 23.0 +/−0.0 | 16.78 +/−0.2 | 2 | 148 | |
| skn-1(zu67) (control RNAi) BHA/DMSO | 23.5 +/−0.5 | 16.66 +/−0.3 | n.s.d | 2 | 147 |
| skn-1(zu67) (daf-2 RNAi) DMSO | 33.5 +/−1.5 | 21.05 +/−0.3 | <0.0001d,e | 2 | 158 |
| skn-1(zu67) (daf-2 RNAi) BHA/DMSO | 45.5 +/−0.5 | 26.00 +/−1.1 | <0.0001d,e,f | 2 | 150 |
| daf-16(mu86) (control RNAi) H2O | 24.0 +/−1.0 | 16.71 +/−0.1 | 2 | 184 | |
| daf-16(mu86) (control RNAi) NAC/H2O | 22.5 +/−0.5 | 16.35 +/−0.1 | n.s.a | 2 | 195 |
| daf-16(mu86) (daf-2 RNAi) H2O | 24.5 +/−0.5 | 16.55 +/−0.1 | n.s.a.b | 2 | 166 |
| daf-16(mu86) (daf-2 RNAi) NAC/H2O | 24.0 +/−0.0 | 16.20 +/−0.1 | n.s.a.b.c | 2 | 186 |
| daf-16(mu86) (control RNAi) DMSO | 24.5 +/−0.5 | 14.68 +/−0.1 | n.s. | 2 | 162 |
| daf-16(mu86) (control RNAi) BHA/DMSO | 23.5 +/−0.5 | 14.62 +/−0.1 | n.s.d | 2 | 176 |
| daf-16(mu86) (daf-2 RNAi) DMSO | 24.5 +/−0.5 | 14.87 +/−0.1 | n.s.d.e | 2 | 180 |
| daf-16(mu86) (daf-2 RNAi) BHA/DMSO | 24.0 +/−0.0 | 14.64 +/−0.1 | n.s.d.e.f | 2 | 171 |
| N2 (control RNAi) | 33.0 +/−0.5 | 22.6 +/− 0.3 | 3 | 350 | |
| N2 (B0513.5 RNAi) | 32.0 +/−1.0 | 22.6 +/− 0.1 | n.s.a | 2 | 240 |
| N2 (daf-2 RNAi) | 69.0 +/−1.0 | 36.4 +/− 1.9 | <0.0001a | 3 | 423 |
| N2 (B0513.5 RNAi/daf-2 RNAi) | 55.0 +/−1.0 | 31.1 +/− 1.1 | <0.0001a,c | 3 | 345 |
| N2 (H2O) | 29.5 +/−0.5 | 20.46 +/−0.3 | 2 | 312 | |
| L-Proline (5 μM) | 33.5 +/−0.5 | 21.65 +/−0.1 | <0.0001i | 2 | 256 |
Controls:
a
(control RNAi) H2O,
b
(control RNAi) NAC/H2O,
c
(daf-2 RNAi) H2O,
d
(control RNAi) DMSO,
e
(control RNAi) BHA/DMSO,
f
(daf-2 RNAi) DMSO,
g
daf-2 (e1370) H2O,
h
daf-2 (e1370) DMSO,
i
(H2O)