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rAed a 3 protein binds to the IgE in mosquito-allergic human serum. Proteins in Ae. aegypti saliva (lane 1) and purified rAed a 3 (lane 2) separated by 10% SDS-PAGE were transferred to nitrocellulose membrane. The membrane was immunoblotted with a pooled mosquito-allergic human serum and incubated with monoclonal anti-human IgE.
