Figure 2.
Effect of CTX on changes in number of vessels and vessel diameters induced by Walker 256 carcinoma cell inoculation. Tumor cells (1 × 106 in 100 µL) were subcutaneously injected into the plantar region of the right hind paw of the rats. CTX (18 µg per rat in 300 µL) or saline (vehicle control) was s.c. administered daily, during 5 days (first injection on day 1, immediately after tumor cell inoculation). (a) The number of vessels and (b) vessel diameters (nm) were determined on the fifth day. (c) Representative histopathological slides of paws injected with Walker 256 carcinoma cells of animals treated with saline (1 and 3) or CTX (2 and 4). Samples were obtained on the fifth day of treatment and stained with monastral blue. (∗) The number of vessels. (→) The vessel diameter. (E), (D), and (H) represent epidermis, dermis, and hypodermis, respectively. Detail of vessel diameters is indicated in (3) and (4) (400x). Each point represents the mean ± SEM of 5 rats. ∗ p < 0.05, significantly different from mean values for saline injected rats at the fifth day after cell injection.